p21-Activated Kinase 1 (PAK1) Can Promote ERK Activation in a Kinase-independent Manner

Abstract

PAK1 plays an important role in proliferation and tumorigenesis, at least partially by promoting ERK phosphorylation of C-RAF (Ser-338) or MEK1 (Ser-298). We observed how that overexpression of a kinase-dead mutant form of PAK1 increased phosphorylation of MEK1/2 (Ser-217/Ser-221) and ERK (Thr-202/Tyr-204), although phosphorylation of B-RAF (Ser-445) and C-RAF (Ser-338) remained unchanged. Furthermore, increased activation of the PAK1 activator Rac1 induced the formation of a triple complex of Rac1, PAK1, and MEK1 independent of the kinase activity of PAK1. These data suggest that PAK1 can stimulate MEK activity in a kinase-independent manner, probably by serving as a scaffold to facilitate interaction of C-RAF.Background: The interaction of PAK1 and the RAF-MEK-ERK cascade is unclear.Results: Overexpression of a kinase-dead mutant form of PAK1 increased phosphorylation of MEK1/2 and ERK. Hyperactivated Rac1 induced the formation of a triple complex of Rac1, PAK1, and MEK1 independent of the kinase activity of PAK1.Conclusion: PAK1 activated MEK-ERK cascade in a kinase-independent manner.Significance: PAK1 might be a scaffold to facilitate interaction of C-RAF and MEK1. PAK1 plays an important role in proliferation and tumorigenesis, at least partially by promoting ERK phosphorylation of C-RAF (Ser-338) or MEK1 (Ser-298). We observed how that overexpression of a kinase-dead mutant form of PAK1 increased phosphorylation of MEK1/2 (Ser-217/Ser-221) and ERK (Thr-202/Tyr-204), although phosphorylation of B-RAF (Ser-445) and C-RAF (Ser-338) remained unchanged. Furthermore, increased activation of the PAK1 activator Rac1 induced the formation of a triple complex of Rac1, PAK1, and MEK1 independent of the kinase activity of PAK1. These data suggest that PAK1 can stimulate MEK activity in a kinase-independent manner, probably by serving as a scaffold to facilitate interaction of C-RAF. Background: The interaction of PAK1 and the RAF-MEK-ERK cascade is unclear. Results: Overexpression of a kinase-dead mutant form of PAK1 increased phosphorylation of MEK1/2 and ERK. Hyperactivated Rac1 induced the formation of a triple complex of Rac1, PAK1, and MEK1 independent of the kinase activity of PAK1. Conclusion: PAK1 activated MEK-ERK cascade in a kinase-independent manner. Significance: PAK1 might be a scaffold to facilitate interaction of C-RAF and MEK1.