A circular 73 kb DNA from the colourless flagellate Astasia longa that resembles the chloroplast DNA of Euglena: restriction and gene map

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

Molecular Cloning. A Laboratory Manual

EJ Wood · Biochemical Education · 1983 · 30.6K citations

Life Sciences, Molecular Biology, Molecular Genetics +13

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

Life Sciences, Molecular Biology, Molecular Genetics +14

A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

Life Sciences, Microbiology, Molecular Biology +13