Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

Life Sciences, Genetics, Microbiology +12

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

Life Sciences, Genetics, Molecular Biology +15

[2] New M13 vectors for cloning

Joachim Messing · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 5.4K citations

Life Sciences, Biotechnology, Molecular Biology +8