Characteristics of cinnamaldehyde nanoemulsion prepared using APV-high pressure homogenizer and ultra turrax

Abstract

This work aims at determining the optimized parameter to prepare cinnamaldehyde nanoemulsion by using high pressure homogenizer (2 passes at 900 bar) and ultra turrax T25 (12000 rpm for 5 min). Thirteen formulation of cinnamaldehyde nanoemulsion obtained by Design Expert software were prepared at a range of oil and surfactant concentration between of 5% and 10% (v/v). Commercial cinnamaldehyde was blended with deionized water and Tween 80 (emulsifier). The responses used in obtaining the optimized condition were droplet size, polydispersity index (PDI) and emulsion stability (ζ-potential). Result showed that nanoemulsion prepared using 5% (v/v) cinnamaldehyde and 5% (v/v) Tween 80 and homogenized using high pressure homogenizer (APV, Germany) has the smallest size of droplet. The response surface plots for droplet size showed that droplet size (diameter, nm) decreased as the concentration of cinnamaldehyde oil and Tween 80 decreased. However ζ-potential value (mV) showed an increment as the cinnamaldehyde oil concentration decreased and Tween 80 increased. The optimum formulation as predicted by response surface methodology in order to produce a stable cinnamaldehyde nanoemulsion was at 5% cinnamaldehyde oil and 7.11% Tween 80. At this optimized conditions the droplet size and ζ-potential values were 56.56 nm and −4.32 mV, respectively.