Construction and characterization of a recombinant esterase with high activity and enantioselectivity to (S)-ketoprofen ethyl ester

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text

Life Sciences, Bioinformatics, Sequence Analysis +14

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

C. Kendall Stover, X. Q. Pham, Alice L. Erwin et al. · Nature · 2000 · 4.6K citations · Full text

Life Sciences, Genetics, Microbiology +13

<i>Escherichia coli</i> maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

Rachel B. Kapust, David S. Waugh · Protein Science · 1999 · 964 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

DNA shuffling of a family of genes from diverse species accelerates directed evolution

Andreas Crameri, Sun-Ai Raillard, Ericka Bermudez et al. · Nature · 1998 · 828 citations

Life Sciences, Genetics, Molecular Biology +13

An evolutionary treasure: unification of a broad set of amidohydrolases related to urease

Liisa Holm, Chris Sander · Proteins Structure Function and Bioinformatics · 1997 · 504 citations

Life Sciences, Biochemistry, Molecular Biology +17