PLoS ONE · 2012 · 107 citations · 77 references
The combined method of cDNA amplification, Illumina sequencing and de novo assembly is suitable for transcriptomic analysis of tiny organs in insects. Through analysis of the transcriptome, genomic features of the primary salivary glands were dissected and biologically important proteins, especially secreted proteins, were predicted. Our findings provide substantial sequence information for the primary salivary glands of whiteflies and will be the basis for future studies on whitefly-plant interactions and virus transmission.
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Mapping and quantifying mammalian transcriptomes by RNA-Seq
A Mortazavi, Brian A. Williams, Kenneth McCue et al. · Nature Methods · 2008 · 14K citations
Functional Genomics, Genetics, Transcriptomics Technology +7
mRNA-Seq whole-transcriptome analysis of a single cell
Fuchou Tang, Cátálin Bárbácioru, Yangzhou Wang et al. · Nature Methods · 2009 · 3.9K citations
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