Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Continuous cultures of fused cells secreting antibody of predefined specificity

Georges Köhler, César Milstein · Nature · 1975 · 17.2K citations

Pharmacy, Cell Biology, Immunology +11

A semi‐empirical method for prediction of antigenic determinants on protein antigens

A. S. Kolaskar, Prasad Tongaonkar · FEBS Letters · 1990 · 1.5K citations

Life Sciences, Biochemistry, Bioinformatics +7

Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh

Asim K. Bej, Donald P. Patterson, Cynthia W. Brasher et al. · Journal of Microbiological Methods · 1999 · 617 citations

Life Sciences, Infectious Diseases, Microbiology +13

Identification of <i>Vibrio parahaemolyticus</i> Strains at the Species Level by PCR Targeted to the <i>toxR</i> Gene

Yung Bu Kim, Jun Okuda, Chiho Matsumoto et al. · Journal of Clinical Microbiology · 1999 · 496 citations · Full text

Life Sciences, Microbiology, Molecular Biology +16