Concepedia
Biochemical and Biophysical Research Communications · 2004 · 149 citations · 18 references
Life SciencesBiochemistryMolecular BiologyProtein EngineeringStructural BiologyProtein Expression EngineeringProtein PurificationRecombinant Protein ExpressionRecombinant Protein ProductionProtein Solubility OptimizationProtein SolubilizationRecombinant Fusion Proteins
18
<i>Escherichia coli</i> maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused
Rachel B. Kapust, David S. Waugh · Protein Science · 1999 · 964 citations · Full text
Life Sciences, Biochemistry, Molecular Biology +16
A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm
Edward R. LaVallie, Elizabeth Diblasio, Sharlotte Kovacic et al. · Nature Biotechnology · 1993 · 931 citations
Life Sciences, Genetics, Molecular Biology +11
The Role of the Thioredoxin and Glutaredoxin Pathways in Reducing Protein Disulfide Bonds in the Escherichia coliCytoplasm
William A. Prinz, Fredrik Åslund, Arne Holmgren et al. · Journal of Biological Chemistry · 1997 · 672 citations · Full text
Life Sciences, Biochemistry, Molecular Biology +15
Strategies for achieving high-level expression of genes in Escherichia coli.
Savvas C. Makrides · Microbiological Reviews · 1996 · 577 citations · Full text
Life Sciences, Biotechnology, Molecular Biology +17
New fusion protein systems designed to give soluble expression inEscherichia coli
Gregory D. Davis, Claude Elisee, Denton M. Newham et al. · Biotechnology and Bioengineering · 1999 · 326 citations