Journal of the American Chemical Society · 2004 · 179 citations · 10 references
PharmacyBiotechnologyMolecular BiologyHemostasisNucleic Acid BiochemistryNucleic Acid ChemistryLigand BindingNucleic Acid LabelingNucleic Acid ModificationOligonucleotide ChemistryThrombin GenerationAptamer ChemistryAptamer EngineeringAptamer RecognitionAptamersChemical Modification Of AptamersLight RegulationThrombin InhibitionThrombin Regulation
"Caged" derivatives of a 15 nucleotide ssDNA anti-thrombin aptamer have been synthesized in which thymidine nucleotides are modified with photolabile protecting groups. One caged thymidine in a key location is enough to completely mask the aptamer's function in respect to their affinity for thrombin and their inhibition of the blood clotting cascade. With light (366 nm) the caging group can be removed, yielding the unmodified active aptamer.
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In vitro selection of RNA molecules that bind specific ligands
Andrew D. Ellington, Jack W. Szostak · Nature · 1990 · 9.7K citations
RNA aptamers as reversible antagonists of coagulation factor IXa
Christopher P. Rusconi, Elizabeth Scardino, Juliana M. Layzer et al. · Nature · 2002 · 512 citations