<i>Arthrobacter aurescens</i> TC1 Atrazine Catabolism Genes <i>trzN</i> , <i>atzB</i> , and <i>atzC</i> Are Linked on a 160-Kilobase Region and Are Functional in <i>Escherichia coli</i>

Abstract

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.