Nucleic Acids Research · 1999 · 37 citations · 17 references
Life SciencesGeneticsMolecular GeneticsGenetic EngineeringGenome EngineeringRecombinationAnimal Cell Line DevelopmentChromosome EngineeringGene MappingGenetic MappingSelectable MarkersTransgene ConstructionCounter-selectable MarkersCre-lox Recombination SystemsMammalian Cell EngineeringRecombinase ProteinsStable Cell Line EngineeringTransgene TargetingTransgenic Cell Line EngineeringTransgenic Cell Lines
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.
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Sex Chromosomes and Sex-linked Genes.
Annals of Internal Medicine · 1968 · 1.3K citations
Continental breakup and the ordinal diversification of birds and mammals
S. Blair Hedges, Patrick Parker, Charles G. Sibley et al. · Nature · 1996 · 535 citations