Publication | Open Access
Unexpected chain-terminating side reaction caused by histidine and acetic anhydride in solid-phase peptide synthesis.
20
Citations
0
References
1989
Year
Solid-phase Peptide SynthesisBioorganic ChemistryPeptide EngineeringMolecular BiologyPeptide SciencePeptide ChemistryChemical BiologyProtein PurificationProtein FoldingProtein ChemistryAcetic AnhydrideBiochemistrySide ReactionSerious Side ReactionChain-terminating Side ReactionBiomolecular EngineeringNatural SciencesPeptide LibraryAc2o Capping ProcedurePeptide SynthesisProtein EngineeringMedicine
Capping is a useful technique to facilitate purification of a crude deprotected peptide in solid-phase peptide synthesis. However, we observed a serious side reaction caused by the Ac2O capping procedure, when it was applied to a synthesis of a peptide containing His. The mechanism of the side reaction was studied.