Journal of Essential Oil Bearing Plants · 2011 · 10 citations · 8 references
Life SciencesBotanyPlant SciencesAnalytical ToxicologyPlant BiochemistryPlant PhysiologyAromatic PlantsMedicinal PlantsPlant ProductsToxic PlantsBioactive ConstituentsCell Viability AssaysEssential Oil AnalysisEssential Oil ToxicityMedicinal Aromatic PlantsToxicity Of Medicinal PlantsToxicology Of Medicinal Plants
Abstract The leaf essential oil of Chromolaena odorata L. (Asteraceae) harvested in Togo were steam-distilled and investigated for chemical composition (GC and GC-MS) and in vitro potential cytotoxic activity on human epidermic cell line HaCat. The chemical composition showed that the main constituents of essential oil sample were respectively β-caryophyllene (25.2 %), germacrene D (18.8 %), and linalool (7.9 %). The in vitro cytotoxicity bioassays on human cell line HaCaT did not revealed any toxicity of C. odorata essential oil up to 3000 μL.mL−1. Pure commercial β-caryophyllene and germacrene D standards appeared almost non-toxic (IC50 >3000 μL.mL−1), proving the major role played by the major components in the overall cell viability showed by the C. odorata oil sample tested in this work.
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