Concepedia

Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Sarah J. R. Staton, Paul V. Jones, Ginger Ku, S. Douglass Gilman, Indu Kheterpal, Mark A. Hayes

The Analyst · 2012 · 35 citations · 14 references

Abstract

Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.

References

14

Electrophoretic and Dielectrophoretic Field Gradient Technique for Separating Bioparticles

Michele D. Pysher, Mark A. Hayes · Analytical Chemistry · 2007 · 160 citations

Engineering, Biosensing Systems, New Device +14

Kinetics and Thermodynamics of Amyloid Assembly Using a High‐Performance Liquid Chromatography–Based Sedimentation Assay

Brian O’Nuallain, Ashwani Kumar Thakur, Angela D. Williams et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 2006 · 147 citations

Bioanalysis, Sedimentation Assay, Protein Misfolding +8

Capillary electrophoresis studies on the aggregation process of β‐amyloid 1‐42 and 1‐40 peptides

Stefania Sabella, Milena Quaglia, Cristina Lanni et al. · Electrophoresis · 2004 · 71 citations

Capillary Electrophoresis, Aggregation Process, Biochemistry +14

Insulator‐based dielectrophoretic separation of small particles in a sawtooth channel

Kang Ping Chen, J. Rafael Pacheco, Mark A. Hayes et al. · Electrophoresis · 2009 · 63 citations

Analysis of monomeric Aβ (1–40) peptide by capillary electrophoresis

Ryan A. Picou, Julia P. Moses, Amber D. Wellman et al. · The Analyst · 2010 · 46 citations