Improving the expression yield of Candida antarctica lipase B in Escherichia coli by mutagenesis

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

Engineering, Hybridisation, Hybridization +13

Quantitative analyses of biochemical kinetic resolutions of enantiomers

Ching Shih Chen, Yoshitaka Fujimoto, Gary Girdaukas et al. · Journal of the American Chemical Society · 1982 · 2.6K citations

Charles J. Sihcite, Engineering, Chemical Analysis +19

A general method of<i>in vitro</i>preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

Russell Higuchi, Barbara Krummel, Randall K. Saiki · Nucleic Acids Research · 1988 · 2.4K citations · Full text

The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica

J. Uppenberg, Mogens Trier Hansen, Shamkant Patkar et al. · Structure · 1994 · 892 citations

Biochemistry, Lipid Resource, Protein X-ray Crystallography +6