Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

Vivo Genetic Engineering, Engineering, Transposon Mutagenesis +10

Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products

Jo Handelsman, Michelle R. Rondon, Sean F. Brady et al. · Chemistry & Biology · 1998 · 1.9K citations · Full text

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

H. Shizuya, Bruce W. Birren, U J Kim et al. · Proceedings of the National Academy of Sciences · 1992 · 1.7K citations · Full text

Genetics, Bacteriology, Bacteriophage +22

Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria

Marta Herrero, Vı́ctor de Lorenzo, Kenneth N. Timmis · Journal of Bacteriology · 1990 · 1.6K citations · Full text

Engineering, Genetics, Bacteriology +22

Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms

Michelle R. Rondon, Paul R. August, Alan D. Bettermann et al. · Applied and Environmental Microbiology · 2000 · 1.2K citations · Full text