Proceedings of the National Academy of Sciences · 1969 · 485 citations · 13 references
Tumor InnervationCell CultureCellular NeurobiologyGliomaNeuroblastoma CellsCellular PhysiologySocial SciencesTumor BiologyNeuro-oncologyNeuroregenerationClonal LinesEpendymaMouse NeuroblastomaNeural CrestNeuroblastsCell BiologyDevelopmental BiologyNeuroanatomyNeuroscienceMedicineNeural Stem Cell
Neuroblastoma cells were cultured using an animal‑culture alternate passage technique, single‑cell plated, and cloned to produce neuronal lines that were assayed for acetylcholinesterase, cholineacetylase, and tyrosine hydroxylase. The clonal neuronal lines retained tumorigenic potential, displayed mature neuronal morphology versus immature tumor cells, and expressed acetylcholinesterase, cholineacetylase, and tyrosine hydroxylase at levels similar to the original tumors, with no significant differences among clones.
Clonal lines of neurons were obtained in culture from a mouse neuroblastoma. The neuroblastoma cells were adapted to culture growth by the animal-culture alternate passage technique and cloned after single-cell plating. The clonal lines retained the ability to form tumors when injected back into mice. A striking morphological change was observed in the cells adapted to culture growth; they appeared as mature neurons, while the cells of the tumor appeared as immature neuroblasts. Acetylcholinesterase and the enzymes for the synthesis of neurotransmitters, cholineacetylase and tyrosine hydroxylase were assayed in the tumor and compared with brain levels; tyrosine hydroxylase was found to be particularly high, as described previously in human neuroblastomas. The three enzymes were found in the clonal cultures at levels comparable to those found in the tumors. Similarly, there were no remarkable differences between the three clones examined.
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Differentiated Rat Glial Cell Strain in Tissue Culture
Philippe Benda, J. Lightbody, Gordon Sato et al. · Science · 1968 · 1.5K citations