FEMS Microbiology Letters · 1999 · 13 citations · 28 references
Life SciencesFood ScienceMicrobiologyAntimicrobial ResistanceBacteriologyMicrobial PhysiologyAnaerobic BacteriologyAntibiotic ResistanceAntibiotic Resistance MechanismsBacterial PhysiologyMicrobial Cell Envelope PhysiologyResistance In Gram-negative BacteriaAntibiotic Resistance Mechanisms In The Cell EnvelopeBacterial Cell Wall PhysiologyCell Envelope CompositionCell Envelope PhysiologyCell Wall-related ResistanceMembrane Fatty Acid CompositionMembrane Fluidity AdaptationResistance Mechanisms In Gram-negative Bacteria
A nisin-resistant strain of Pectinatus frisingensis (Nis-5000) to high nisin concentration (5000 IU ml−:1) was isolated. The nisin resistance phenotype of strain Nis-5000 was stable after five passages through nisin-free medium. The involvement of the cell envelope in nisin resistance was investigated. EDTA treatment of Nis-5000 strain cells did not render them sensitive to the bacteriocin, suggesting that the lipopolysaccharide layer was not involved in nisin resistance. The Nis-5000 strain shows a significantly different fatty acid composition compared with that of the wild-type strain. The modifications occurred mainly in C17:1 (P<0.05) and C18:cyclo (P<0.05), suggesting the involvement of membrane fluidity in preventing nisin's effect.
28