Destabilization of pea lectin by substitution of a single amino acid in a surface loop

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Molecular Cloning. A Laboratory Manual

EJ Wood · Biochemical Education · 1983 · 30.6K citations

Molecular Biological Method, Natural Sciences, Genetics +8

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

Electrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands

Ben Lugtenberg, Jos Meijers, R.C. Peters et al. · FEBS Letters · 1975 · 1.4K citations · Full text

Response of a Protein Structure to Cavity-Creating Mutations and Its Relation to the Hydrophobic Effect

A.E. Sauer-Eriksson, W.A. Baase, X. J. Zhang et al. · Science · 1992 · 939 citations

Biophysical Modeling, Protein Assembly, Biomolecular Structure Prediction +18