Extensive N-Glycosylation Reduces the Thermal Stability of a Recombinant Alkalophilic Bacillus α-Amylase Produced in Pichia pastoris

The Mechanism of Irreversible Enzyme Inactivation at 100°C

Tim J. Ahern, Alexander M. Klibanov · Science · 1985 · 446 citations

Life Sciences, Biochemistry, Molecular Biology +17

Crystal Structure of Calcium-depletedBacillus licheniformisα-amylase at 2.2 Å Resolution

Mischa Machius, Georg Wiegand, Robert Huber · Journal of Molecular Biology · 1995 · 321 citations

Life Sciences, Biochemistry, Microbiology +14

The Amino Acid at the X Position of an Asn-X-Ser Sequon Is an Important Determinant of N-Linked Core-glycosylation Efficiency

Susan H. Shakin-Eshleman, Steven L. Spitalnik, Lakshmi Kasturi · Journal of Biological Chemistry · 1996 · 311 citations · Full text

Life Sciences, Molecular Biology, Molecular Virology +14

Glycosylation of <i>Pichia pastoris</i> ‐derived proteins

Roger K. Bretthauer, Francis Castellino · Biotechnology and Applied Biochemistry · 1999 · 300 citations · Full text

Life Sciences, Biochemistry, Microbiology +15

Advances in the use of Pichia pastoris for high-level gene expression

Mike Romanos · Current Opinion in Biotechnology · 1995 · 251 citations

Life Sciences, Biological Engineering, Biotechnology +14