Advances in the use of Pichia pastoris for high-level gene expression

High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene

Jeff J. Clare, F. B. Rayment, S. Ballantine et al. · Nature Biotechnology · 1991 · 393 citations · Full text

Microbial Toxin, Biosynthesis, Engineering +6

High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris

J M Cregg, J Tschopp, Cathy A. Stillman et al. · Nature Biotechnology · 1987 · 336 citations

Engineering, Natural Sciences, Biochemical Engineering +9

Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies

Jeffrey J. Clare, Michael A. Romanes, F. B. Rayment et al. · Gene · 1991 · 331 citations

Engineering, Genetics, Biotechnology +10

Rapid Selection Using G418 of High Copy Number Transformants of Pichia pastoris for High–level Foreign Gene Expression

Carol A. Scorer, Jeffrey J. Clare, W. Richard McCombie et al. · Nature Biotechnology · 1994 · 307 citations

Engineering, Genetics, Biotechnology +7

High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast, Pichia Pastoris

J Tschopp, Genadie G. Sverlow, Ryszard Kosson et al. · Nature Biotechnology · 1987 · 247 citations

Biosynthesis, Engineering, Cellular Enzymology +11