Concepedia

P <sub>BAD</sub> -Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in <i>Pseudomonas</i> and <i>Burkholderia</i> spp. and Other Bacteria

Dongru Qiu, F. Heath Damron, Takehiko Mima, Herbert P. Schweizer, Hongwei D. Yu

Applied and Environmental Microbiology · 2008 · 280 citations · 30 references

DOI

Concepts

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

References

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