P _BAD -Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in <i>Pseudomonas</i> and <i>Burkholderia</i> spp. and Other Bacteria

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.