Japanese Journal of Cancer Research · 1989 · 10 citations · 13 references
MedicineGeneticsMolecular GeneticsCancer GeneticsMolecular OncologyOncogenesLymphoid NeoplasmsLymphoid Tissue PathologyLymphoid TumorsMutation AnalysisOncogene MutationsRas Family OncogenesActivating Point MutationsCodon 61 MutationsDna In Situ HybridizationDna ProbesGenomic Dna AnalysisOncogenic Ras MutationsPoint Mutation AnalysisPoint Mutations
Twenty-three cases of non-Hodgkin's lymphoma (NHL) were analyzed for expression of ras genes by in situ hybridization utilizing biotinylated DNA probes. Increased expression of Ki-ras, Ha-ras and N-ras genes was observed in 12 cases, 6 cases and 1 case of NHL, respectively. Genomic DNA extracted from these 23 cases of NHL was region-specifically amplified by means of polymerase chain reaction to examine the presence of point mutations at the 12th, 13th and 61st codons of Ki-, Ha- and N-ras genes. Dot hybridization assays with appropriate oligonucleotide probes showed no evidence of point mutation in any case of NHL examined. These results indicate that increased expression of ras genes in NHL is not associated with ras gene activation by point mutation.
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Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes
Concepción Almoguera, Darryl Shibata, Kathleen Forrester et al. · Cell · 1988 · 2.1K citations
Prevalence of ras gene mutations in human colorectal cancers
Johannes L. Bos, Eric R. Fearon, Stanley R. Hamilton et al. · Nature · 1987 · 1.9K citations
Detection of high incidence of K-ras oncogenes during human colon tumorigenesis
Kathleen Forrester, Concepción Almoguera, Kyuhyung Han et al. · Nature · 1987 · 1.1K citations
Medicine, Cancer Biology, Oncology +12