Biotechnology and Bioengineering · 1995 · 38 citations · 15 references
Life SciencesPlant BiologyPlant SciencesPhysiological EcologyPlant BiochemistryPlant PhysiologyBioprocess KineticsCell Growth KineticsContinuous CultureDissolved Oxygen DynamicsOxygen EvolutionAir Saturation ApproachConcentrationGas FlowOxygen Concentration EffectsOxygen DeliverySaturationStirred Tank Gas-liquid ReactorsStirred Tank Reactors
The relation between dissolved oxygen (DO) and the ajmalicine production rate of Catharanthus roseus was investigated in 15-L tank reactors at constant stirrer speed and gas flow rate. Below a DO concentration of 29% of air saturation the ajmalicine production rate was less than 0.06 micromol/g/d. Above a DO of 43% the ajmalicine production rate was constant at 0.21 micromol/g/d. Between a DO of 29% and 43% there was a strong relation between the ajmalicine production rate and the DO concentration. After a period of at least 12 days at DO <or=29% the culture lacked the ability to adapt to a DO >or=57%. A kinetic equation is proposed for the relation between DO and the specific ajmalicine production rate.
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Plant cell reactors—A perspective
Amulya K. Panda, Saroj Mishra, Virendra S. Bisaria et al. · Enzyme and Microbial Technology · 1989 · 109 citations