Review of Scientific Instruments · 1996 · 72 citations · 10 references
Natural SciencesBiomedical EngineeringPhysicsBiomedical OpticsMicroelectromechanical SystemsOpticsAtomic Force MicroscopyForce SensingForce SensorsOptical InstrumentsScanning Probe MicroscopyNear-field Optical MicroscopyNear-field OpticsNear-field ScanningNear-field Scanning Optical MicroscopyNormal ForceNormal Force SensingScanning Near-field Optical MicroscopyShear Force Measurement
We present a near-field scanning optical microscope (NSOM) which fully integrates near-field and far-field optics with scanned probe microscopy (SPM). The instrument incorporates a novel, flat scanning system that allows the NSOM/SPM head to be placed directly on the sample stage of any upright or inverted optical microscope and fully integrates the two microscopes. The near-field optical microscope that is designed around this flat scanner utilizes cantilevered optical force sensing probes that we have developed. Thus, the instrument can simultaneously image in a number of topographic and optical modes. The normal force sensing capability of our probes and microscope provides a significant advantage over straight optical fibers and the shear force detection techniques that are employed. The piezoelectric scanner incorporates X, Y, and Z scan ranges of over 40 μm into a flat stage less than 7 mm high. The scanning stage also provides for inertial translation of a sample over many millimeters. This completely and transparently integrates the technologies of near-field optics and other scanned probe microscopies with conventional far-field optical microscopy. Furthermore, the axial positioning capability of the flat scanner enhances the characteristics of the far-field microscope by allowing for three-dimensional optical sectioning that is required in techniques such as confocal imaging, nonlinear optical microscopies, and charge coupled device (CCD) imaging. Finally, the X, Y, and Z precision movements also have the potential for being used in optical tweezer applications.
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Breaking the Diffraction Barrier: Optical Microscopy on a Nanometric Scale
Eric Betzig, J. K. Trautman, T.D. Harris et al. · Science · 1991 · 1.5K citations
Development of a 500 Å spatial resolution light microscope
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