Synthesis of docosahexaenoic acid‐rich triglyceride with immobilized<i>Chromobacterium viscosum</i> lipase

Abstract

Abstract Docosahexaenoic acid (DHA) in the free fatty acid (FFA) derived from enzymically hydrolyzed tuna oil was concentrated by partial titration and precipitation of other FFA as sodium salts with acetone. A triglyceride containing up to 46.2% DHA was synthesized from the DHA‐rich glyceride mixture and FFA by use of an immobilized Chromobacterium viscosum lipase.