Bioscience Biotechnology and Biochemistry · 2002 · 48 citations · 13 references
Life SciencesMicrobiologyMolecular BiologyEnzymologyMarine MicrobiologyMicrobial GeneticsMarine Enzyme BiotechnologyMarine Microbial BiotechnologyMarine Microbial GeneticsProteolytic EnzymesProteolytic ProcessingBacterial Protease ActivityBacterial ProteasesExtracellular ProteasesMarine ProteasesMicrobial ProteasesProtease Activity AssaysProtease BiologyProtease Production
The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.
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