Concepedia

Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression

Tamar Unger, Yossi Jacobovitch, Ada Dantes, Reut Bernheim, Yoav Peleg

Journal of Structural Biology · 2010 · 261 citations · 24 references

DOI

Concepts

References

24

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Mamie Z. Li, Stephen J. Elledge · Nature Methods · 2007 · 979 citations

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A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

Edward R. LaVallie, Elizabeth Diblasio, Sharlotte Kovacic et al. · Nature Biotechnology · 1993 · 931 citations

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RF cloning: A restriction-free method for inserting target genes into plasmids

F. van den Ent, Jan Löwe · Journal of Biochemical and Biophysical Methods · 2006 · 643 citations

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Integration of PCR Fragments at Any Specific Site within Cloning Vectors without the Use of Restriction Enzymes and DNA Ligase

Martin Geiser, Régis Cèbe, Delia Drewello et al. · BioTechniques · 2001 · 250 citations · Full text

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An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes

Ralf D. Kirsch, Etienne Joly · Nucleic Acids Research · 1998 · 233 citations · Full text

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