Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocellulose

Jan Kyhse‐Andersen

Journal of Biochemical and Biophysical Methods

Biopolymer GelCapillary ElectrophoresisRapid TransferPolyelectrolyte GelImmobilized Enzyme +6

Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR

Thomas D. Schmittgen, Brian A. Zakrajsek

Journal of Biochemical and Biophysical Methods

EngineeringQuantitative Rt-pcrReporter Gene AssayMedicineGenetics +12

Principles of Fluorescence Spectroscopy

Mahendra Kumar Jain

Journal of Biochemical and Biophysical Methods

Fluorescence MicroscopyMolecular SpectroscopySpectroscopyPhotophysical PropertyBiophysics +1

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

Bengt Bjellqvist, Kristina Ek, Pier Giorgio Righetti, +4

Journal of Biochemical and Biophysical Methods

EngineeringApplied PhysicsElectrophysiologyIsoelectric FocusingImmobilized Ph Gradients +2

Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances

Hisatoshi Kaneko, Takashi Kawana, Eiko Fukushima, +1

Journal of Biochemical and Biophysical Methods

EngineeringBiotechnologyBiochemical EngineeringSynthetic BiologyBiological Substances +4

RF cloning: A restriction-free method for inserting target genes into plasmids

F. van den Ent, Jan Löwe

Journal of Biochemical and Biophysical Methods

Rf CloningGeneticsMolecular BiologyGenetic EngineeringTarget Genes +8

Real-time turbidimetry of LAMP reaction for quantifying template DNA

Yasuyoshi Mori, Masataka Kitao, Norihiro Tomita, +1

Journal of Biochemical and Biophysical Methods

Natural SciencesLamp ReactionOligonucleotideDna ReplicationMolecular Biology +3

In the absence of catalytic metals ascorbate does not autoxidize at pH 7: ascorbate as a test for catalytic metals

Garry R. Buettner

Journal of Biochemical and Biophysical Methods

BiochemistryCatalytic MetalsNatural SciencesBioactive MetalSpectroelectrochemistry +4

2-NBDG as a fluorescent indicator for direct glucose uptake measurement

Chenhui Zou, Yajie Wang, Zhufang Shen

Journal of Biochemical and Biophysical Methods

BiochemistryInsulin ManagementBioanalysisDiabetesPhysiology +5

Sensitive assay of hydroxyl free radical formation utilizing high pressure liquid chromatography with electrochemical detection of phenol and salicylate hydroxylation products

Robert A. Floyd, Julia Watson, Peter K. H. Wong

Journal of Biochemical and Biophysical Methods

BiochemistryElectrochemical DetectionMedicineSensitive AssayRadical (Chemistry) +6