Multiplexed Protein Arrays Enabled by Polymer Pen Lithography: Addressing the Inking Challenge

Abstract

High-end printer: A novel way of using a polymer pen lithography (PPL) array mould to localize different inks on the pens of a PPL array has been developed. This technique allows for rapid pen inking and subsequent generation of multiplexed patterns of up to 150 000 nano- and microscale protein features per second (see picture). It is a general approach, which in principle can be extended to any substance that can be transported by PPL.