Concepedia

A rapid purification method for calf thymus casein kinase II

Rubén O. Zandomeni, M.C. Zandomeni, Roberto Weinmann

FEBS Letters · 1988 · 18 citations · 19 references

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Concepts

Abstract

We report here the rapid purification to homogeneity of a cyclic nucleotide-independent protein kinase sensitive to 5'6-dichloro-1-beta-D-ribofuranozylbenzimidazole (DRB), identical to the previously described casein kinase II, from lyophilized calf thymus by chromatography on phosphocellulose and Mono-Q FPLC columns.

References

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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Casein kinase type II is involved in the inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription.

Rubén O. Zandomeni, M.C. Zandomeni, David Shugar et al. · Journal of Biological Chemistry · 1986 · 294 citations · Full text

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Purification and properties of calf thymus casein kinases I and II.

Michael Dahmus · Journal of Biological Chemistry · 1981 · 156 citations · Full text

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