Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post-Amplification Labelling”

Abstract

Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330-mer fragment with T7 RNA polymerase.