Concepedia

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

Teegan A Delli-Bovi, Maroya D. Spalding, Sean T. Prigge

BMC Biotechnology · 2010 · 11 citations · 28 references

DOIFull text

Open access

Abstract

The strategy described in our report provides a simple and effective method for the production of [35S]-biotin in E. coli based on affinity chromatography.

References

28

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15K citations · Full text

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Michael D. Abràmoff, Paulo Magalhães, Sunanda J. Ram · Utrecht University Repository (Utrecht University) · 2004 · 11.9K citations · Full text

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Construction of <i>Escherichia coli</i> K‐12 in‐frame, single‐gene knockout mutants: the Keio collection

Tomoya Baba, Takeshi Ara, Miki Hasegawa et al. · Molecular Systems Biology · 2006 · 8.1K citations · Full text

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Assembly of Iron-Sulfur Clusters

Limin Zheng, Valerie L. Cash, Dennis H. Flint et al. · Journal of Biological Chemistry · 1998 · 642 citations · Full text

Acetyl coenzyme A carboxylase.

Lanĕ, Joel Moss, Polakis Se · PubMed · 1974 · 219 citations

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