DETERMINATION OF GLYOXAL, METHYLGLYOXAL, AND DIACETYL IN SELECTED BEER AND WINE, BY HPLC WITH UV SPECTROPHOTOMETRIC DETECTION, AFTER DERIVATIZATION WITH o-PHENYLENEDIAMINE

Abstract

The α-diketones glyoxal, methylglyoxal, and diacetyl were determined in selected beer and wine using a procedure involving the use of C18 solid phase extraction columns to remove interferences and derivatization of the compounds with o-phenylenediamine to form quinoxalines, which are separated by HPLC and detected using UV spectrophotometric detection.