Stabilization of enzymes (d-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques

Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes

JoséM. Guisán · Enzyme and Microbial Technology · 1988 · 594 citations

Engineering, Biochemical Engineering, Biotechnology +5

Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method

Roberto Fernández‐Lafuente, Cristina M. Rosell, V. Rodríguez et al. · Enzyme and Microbial Technology · 1993 · 255 citations

Protein Immobilization, Biochemistry, Protein Folding +9

Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment

Rosa M. Blanco, Juan J. Calvete, JoséM. Guisán · Enzyme and Microbial Technology · 1989 · 197 citations

Biochemistry, Protein Folding, Natural Sciences +9

Strategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents

Roberto Fernández‐Lafuente, Cristina M. Rosell, V. Rodríguez et al. · Enzyme and Microbial Technology · 1995 · 165 citations

Biochemistry, Enzyme Stabilization, Enzyme Catalysis +4

Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives

Rosa M. Blanco, JoséM. Guisán · Enzyme and Microbial Technology · 1989 · 156 citations

Trypsin-agarose Derivatives, Multipoint Covalent Attachment, Biochemistry +10