JoséM. Guisán

Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes

JoséM. Guisán · Enzyme and Microbial Technology · 1988 · 594 citations

Engineering, Biochemical Engineering, Biotechnology +5

Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment

Rosa M. Blanco, Juan J. Calvete, JoséM. Guisán · Enzyme and Microbial Technology · 1989 · 197 citations

Biochemistry, Protein Folding, Natural Sciences +9

Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives

Rosa M. Blanco, JoséM. Guisán · Enzyme and Microbial Technology · 1989 · 156 citations

Trypsin-agarose Derivatives, Multipoint Covalent Attachment, Biochemistry +10

Protecting effect of competitive inhibitors during very intense insolubilized enzyme-activated support multipoint attachments: trypsin (amine)-agarose (aldehyde) system

Rosa M. Blanco, JoséM. Guisán · Enzyme and Microbial Technology · 1988 · 59 citations

Medicinal Chemistry, Biochemistry, Natural Sciences +8

Additional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde

Roberto Fernández‐Lafuente, Cristina M. Rosell, Gregorio Álvaro et al. · Enzyme and Microbial Technology · 1992 · 56 citations

Controlled Chemical Modification, Medicinal Chemistry, Additional Stabilization +10