Simultaneous determination of trichothecene micotoxins, ochratoxin A, and zearalenone in grain and products of its processing, feed premixes, and meat by gas chromatography

Abstract

A rapid and simple method is developed for the simultaneous determination of ochratoxin A, toxin T-2, deoxynivalenol, and zearalenone by gas chromatography with electron capture detection; the method is applicable to grain, products of its processing, feed premixes, and meat in concentration ranges 0.05–5 mg/kg (0.01–2 mg/kg for ochratoxin A and 0.03–3 mg/kg for T-2). The micotoxins were extracted from samples with acetonitrile; the extracts were purified by the QuEChERS method; and derivatives of target analytes were obtained with trifluoroacetic anhydride. The overall duration of analysis was 1–1.5 h; the relative standard deviation of the results did not exceed 8%.