FEBS Letters · 1999 · 14 citations · 22 references
Environmental SignalingMolecular RegulationGeneticsImmunologyMolecular BiologyGene Regulatory NetworkInterferon‐resistant Daudi SublineTranscriptional RegulationProtein ExpressionDaudi Res CellsIfn‐resistant Daudi SublineInitiator ElementCell SignalingMolecular SignalingDefective BindingMedicineGene ExpressionEpigenetic RegulationCell BiologyTranscription RegulationGene FunctionCytokineSignal TransductionNatural SciencesGene RegulationCellular BiochemistryTranscription FactorsIfn Resistance
To investigate mechanisms of interferon (IFN) resistance, we have established an IFN‐resistant Daudi subline (Daudi res ), which is 1×10 4 times more resistant to IFN‐α than parental cells. Among the IFN‐inducible genes examined, only ICE mRNA expression was deficient in Daudi res cells. We then analyzed the regulatory mechanisms of ICE transcription, and found that IFN‐induced activation of the ICE promoter was dependent on the binding of IRFs to its initiator (Inr) element. Inr binding of IRFs was markedly diminished in Daudi res cells, and forced expression of IRF‐1 was able to activate the ICE promoter to the level of parental cells. These results suggest that IRFs and their target genes, as represented by ICE in this study, are involved in IFN resistance.
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