Concepedia

A simple and effective strategy for solving the problem of inclusion bodies in recombinant protein technology: His-tag deletions enhance soluble expression

Shaozhou Zhu, Cuiyu Gong, Lu Ren, Xingzhou Li, Dawei Song, Guojun Zheng

Applied Microbiology and Biotechnology · 2012 · 32 citations · 27 references

Concepts

Effective StrategyEngineeringProtein ExpressionProtein AssemblyInclusion BodiesProtein FoldingNatural SciencesRecombinant Protein TechnologyMolecular BiologyDna ReplicationSynthetic BiologyProtein EngineeringProtein RefoldingProteomicsMulti-protein AssemblyBiomolecular EngineeringProtein Purification

References

27

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Erich Hochuli, Heinz Döbeli, Alfred Schacher · Journal of Chromatography A · 1987 · 1.1K citations

Biochemistry, Natural Sciences, Peptide Engineering +7

Evaluation of comparative protein modeling by M<scp>ODELLER</scp>

Andrej Săli, Liz Potterton, Yuan Feng et al. · Proteins Structure Function and Bioinformatics · 1995 · 1.1K citations

Strategies for optimizing heterologous protein expression in Escherichia coli

Gerhard Hannig, Savvas C. Makrides · Trends in biotechnology · 1998 · 485 citations

Protein Expression, Molecular Biology, Escherichia Coli +2

Refolding of recombinant proteins

Eliana De Bernardez Clark · Current Opinion in Biotechnology · 1998 · 474 citations

Recombinant Proteins, Protein Folding, Natural Sciences +6

Complete Genome Sequence of an Aerobic Hyper-thermophilic Crenarchaeon, Aeropyrum pernix K1

Yutaka Kawarabayasi · DNA Research · 1999 · 426 citations · Full text