On extraction of acyl and alkyl dihydroxyacetone phosphate from incubation mixtures

Abstract

Abstract Chloroform‐methanol mixture was shown to extract acyl and alkyl dihydroxyacetone phosphate from enzyme incubation mixtures. However, when the lipid extract was washed with water to remove nonlipid materials, 70–80% of acyl and alkyl dihydroxyacetone phosphate were lost in the aqueous phase. It was shown that, keeping the pH low (<2.5) during the partition of lipids by the Bligh and Dyer method, most (>95%) of the acyl and alkyl dihydroxyacetone phosphate were recovered in the chloroform‐rich phase. n‐Butanol was shown to extract 80–90% of these lipids from incubation mixtures at neutral pH.