Journal of the Science of Food and Agriculture · 1987 · 36 citations · 9 references
AgricultureCrop SciencesPlant SciencesCereal ScienceCrop PhysiologyGermplasm EvaluationCereal Crop PhysiologyCereal EnzymologyCereal Grain ChemistryCrop Stress DetectionPlant Stress DetectionAssayCereal Crop PathologyCrop Damage DetectionFlour Quality AssessmentGluten ProteinsGrain Damage AnalysisSedimentation TestSedimentation Velocity
Abstract Lines of three varieties of New Zealand wheat (cv. Aotea, Oroua and Kopara) damaged by wheat‐bug were investigated. Hydrolysis of gluten proteins was shown to be of the endo‐ rather than the exo‐proteolytic type. Electrophoresis revealed that the bug protease had a high specificity for the high molecular weight (HMW) glutenin subunits. An autolytic assay method was developed based on the decrease in sodium dodecyl sulphate (SDS)‐sedimentation volume for bug‐damaged flours when they were incubated in distilled water for 30 min at 37°C. This method was specific for bug damage and exhibited no interference from other grain defects such as heat damage, field sprouting and laboratory germination.
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The determination of amino-acids with ninhydrin
E. W. Yemm, E. C. Cocking, R. E. Ricketts · The Analyst · 1955 · 2.3K citations