PLoS ONE · 2013 · 11 citations · 20 references
Life SciencesMolecular BiologyNeuroscienceMolecular NeurobiologyMolecular NeuroscienceStructural BiologyAmyloid FormationNeurotransmissionProtein DomainsProtein Interaction DomainsProtein StructureScaffold ProteinsPeptide Binding AffinityPhosphotyrosine Binding DomainsProtein Binding DomainsProtein ScaffoldingProtein-protein Interaction DomainsStructure SolutionSynaptic Protein InteractionsSynaptic Scaffolding Proteins
AIDA1 links persistent chemical signaling events occurring at the neuronal synapse with global changes in gene expression. Consistent with its role as a scaffolding protein, AIDA1 is composed of several protein-protein interaction domains. Here we report the NMR structure of the carboxy terminally located phosphotyrosine binding domain (PTB) that is common to all AIDA1 splice variants. A comprehensive survey of peptides identified a consensus sequence around an NxxY motif that is shared by a number of related neuronal signaling proteins. Using peptide arrays and fluorescence based assays, we determined that the AIDA1 PTB domain binds amyloid protein precursor (APP) in a similar manner to the X11/Mint PTB domain, albeit at reduced affinity (∼10 µM) that may allow AIDA1 to effectively sample APP, as well as other protein partners in a variety of cellular contexts.
20