Concepedia

A general protocol for the generation of Nanobodies for structural biology

Els Pardon, Toon Laeremans, Sarah Triest, Søren G. F. Rasmussen, Alexandre Wohlkönig, A. Ruf, Serge Muyldermans, Wim G. J. Hol, Brian K. Kobilka, Jan Steyaert

Nature Protocols · 2014 · 812 citations · 60 references

DOI

Concepts

References

60

Naturally occurring antibodies devoid of light chains

C. Hamers‐Casterman, T. Atarhouch, Serge Muyldermans et al. · Nature · 1993 · 3.2K citations

, , +14

Crystal structure of the β2 adrenergic receptor–Gs protein complex

Søren G. F. Rasmussen, Brian T. DeVree, Yaozhong Zou et al. · Nature · 2011 · 3.1K citations · Full text

, , +12

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Piotr Chomczyński, Nicoletta Sacchi · Nature Protocols · 2006 · 2.1K citations

, , +12

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

Søren G. F. Rasmussen, Hee‐Jung Choi, Juan José Fung et al. · Nature · 2011 · 1.7K citations · Full text

, , +6

Conformational biosensors reveal GPCR signalling from endosomes

Roshanak Irannejad, Jin C. Tomshine, Jon R. Tomshine et al. · Nature · 2013 · 840 citations · Full text

, , +12