Biotechnology and Bioengineering · 1996 · 253 citations · 13 references
Life SciencesBiochemistryMaterials ScienceBiocatalysisEnzymologySurface ScienceBiomolecular InterfacesEnzyme FunctionEnzyme Surface EngineeringEnzyme-substrate RecognitionProtein AdsorptionRedox EnzymesAdsorbed Protein LayersGlucose OxidaseOxidase CatalysisProtein Adsorption To BiomaterialsProtein FilmsProtein-based FilmsProtein-surface InteractionsSupramolecular Nanocomposites
Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H(2)O(2)) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H(2)O(2)/DA67 was demonstrated successfully with this supramolecular system.
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