Concepedia

Optimization of temperature–glycerol–pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris

Mehmet İnan, Vijay Chiruvolu, Kent M. Eskridge, George P. Vlasuk, Kenneth Dickerson, Scott Brown, Michael M. Meagher

Enzyme and Microbial Technology · 1999 · 78 citations · 24 references

References

24

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

Life Sciences, Biochemistry, Molecular Biology +10

Statistics for Experimenters

Sidney Addelman, G. E. P. Box, W. G. Hunter et al. · Technometrics · 1979 · 6.5K citations

Response Surface Methodology

Raymond H. Myers, Douglas C. Montgomery · IIE Transactions · 1996 · 3.8K citations

Computing, Computational Engineering, Algorithms +13

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

Koti Sreekrishna, Robert G. Brankamp, Keith E. Kropp et al. · Gene · 1997 · 437 citations

Life Sciences, Biotechnology, Molecular Biology +16

High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene

Jeff J. Clare, F. B. Rayment, S. Ballantine et al. · Nature Biotechnology · 1991 · 393 citations · Full text

Life Sciences, Biotechnology, Microbiology +4