Journal of the American Oil Chemists Society · 1955 · 61 citations · 9 references
Life SciencesBiochemistryToxicologyCellular MetabolismFood AnalysisMetabolismFatty Acid ChemistryFatty Acid OxidationLipid MetabolismLipid OxidationLipid PeroxidationOxidation AnalysisFat Oxidation ProductsFatty Acid Methyl EstersFatty AcidsLight-induced Lipid OxidationLipid Oxidation ProductsOxidative Damage In LipidsPeroxide FormationThiobarbituric Acid Reactive Substances
Summary The thiobarbituric acid (TBA) reaction for fatty acid oxidation has been compared with Lundberg and Chipault's method for peroxides, the Kreis test for aldehydes, and with the degree of conjugation, using fatty acid esters exposed to ultraviolet light for various periods. The TBA test paralleled the other methods for methyl linolenate and methyl linoleate but was essentially negative for methyl oleate oxidation. The sensitivity of the TBA test for linolenate was 30–80 times that for linoleate at the same peroxide values. The TBA test appears to be a reliable method of estimating the oxidation products of linolenic and linoleic acids in tissues and other biological material.
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