Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system

Cell-free translation reconstituted with purified components

Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari et al. · Nature Biotechnology · 2001 · 2K citations

Protein Biosynthesis, Synthetic Biology, Cellular Biochemistry +4

Synthesizing life

Jack W. Szostak, David P. Bartel, P. L. Luisi · Nature · 2001 · 1.6K citations · Full text

A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield

Alexander S. Spirin, Vladimir Baranov, Lyubov A. Ryabova et al. · Science · 1988 · 598 citations

Viral Replication, Engineering, Peptide Engineering +19

<i>In Vitro</i> Selection of Highly Modified Cyclic Peptides That Act as Tight Binding Inhibitors

Yollete V. Guillén Schlippe, Matthew C. T. Hartman, Kristopher Josephson et al. · Journal of the American Chemical Society · 2012 · 240 citations · Full text

Peptide Engineering, Protease Thrombin, Molecular Biology +20

Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

Norikazu Ichihashi, Kimihito Usui, Yasuaki Kazuta et al. · Nature Communications · 2013 · 186 citations · Full text

Biology, Natural Sciences, Directed Evolution +9