Concepedia

Development of a Bicistronic Vector Driven by the Human Polypeptide Chain Elongation Factor 1α Promoter for Creation of Stable Mammalian Cell Lines That Express Very High Levels of Recombinant Proteins

Stephen M. Hobbs, Sarawut Jitrapakdee, John C. Wallace

Biochemical and Biophysical Research Communications · 1998 · 256 citations · 21 references

DOI

Concepts

References

21

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251K citations

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pEF-BOS, a powerful mammalian expression vector

Seiichi Mizushima, Shigekazu Nagata · Nucleic Acids Research · 1990 · 1.6K citations · Full text

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Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

Orna Elroy‐Stein, Thomas R. Fuerst, Bernard Moss · Proceedings of the National Academy of Sciences · 1989 · 404 citations · Full text

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Bicistronic Vector for the Creation of Stable Mammalian Cell Lines that Predisposes All Antibiotic-Resistant Cells to Express Recombinant Protein

Stephen Edward Rees, Jim Coote, Jenny Stables et al. · BioTechniques · 1996 · 284 citations · Full text

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Use of the human elongation factor 1α promoter as a versatile and efficient expression system

Dong Wan Kim, Taichi Uetsuki, Yoshito Kaziro et al. · Gene · 1990 · 282 citations

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