Proceedings of the National Academy of Sciences · 1997 · 114 citations · 48 references
Life SciencesGeneticsMolecular GeneticsDna ReplicationGenome OrganizationGenomicsCis-regulatory ElementsDna Replication ControlReplication In EukaryotesReplication InitiationReplication OriginsYeast GeneticsAutonomously Replicating Sequence ElementsCis-acting Replication ElementsOrigin Recognition ComplexOrigin Recognition SequencesReplication Control ElementsReplication Origin ActivitySequence Determinants Of Replication Origins
Autonomously replicating sequence (ARS) elements, which function as the cis-acting chromosomal replicators in the yeast Saccharomyces cerevisiae, depend upon an essential copy of the 11-bp ARS consensus sequence (ACS) for activity. Analysis of the chromosome III replicator ARS309 unexpectedly revealed that its essential ACS differs from the canonical ACS at two positions. One of the changes observed in ARS309 inactivates other ARS elements. This atypical ACS binds the origin recognition complex efficiently and is required for chromosomal replication origin activity. Comparison of the essential ACS of ARS309 with the essential regions of other ARS elements revealed an expanded 17-bp conserved sequence that efficiently predicts the essential core of ARS elements.
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