Chromatographie analysis of seed oils. Fatty acid composition of castor oil

Abstract

Abstract The fatty acid composition of a number of domestic and foreign castor oils was determined by consecutive column and gas‐liquid Chromatographic analysis. After saponification of the oils and removal of the unsaponifiables, the nonhydroxy, monohydroxy, and dihydroxy acids were fractionated by partition chromatography on silicic acid. The amount of acid in each fraction was determined by titration or weighing. Gravimetric data were in good agreement with the titrimetric data. The acids obtained by saponification were converted to methyl esters with diazomethane and similarly subjected to partition chromatography. The methyl esters from various fractions were analyzed by gas‐liquid chromatography. Components were tentatively identified by their comparative retention times and confirmed